Ọtụtụ n'ime ihe ngwọta nchekwa nje a na-ahụkarị nwere nnu guanidine (guanidine isothiocyanate ma ọ bụ guanidine hydrochloride), nke bụ protein denaturants maka cell lysis n'oge mmịpụta nucleic acid, yana nwekwara ike ime ka nje ghara ịrụ ọrụ.Otú ọ dị, usoro nnu guanidine enweghị ike ichekwa viral nucleic acid n'ime ụlọ okpomọkụ, na-ebute ngwa ngwa nbibi.
Na nzaghachi nye nsogbu ndị a dị n'elu, ihe ngwọta nchekwa nje nnu na-abụghị guanidine nke JanMa Gene mepụtara nwere ọrụ nke inactivation virus na ichekwa sample.Enwere ike ịchekwa acid nucleic nje na ụlọ okpomọkụ maka awa 24 na-enweghị mmebi, nke ndị ahịa kwadoro n'otu ọnụ.
【Oji ngwaahịa】 A na-eji maka mkpokọta nje virus, ichekwa, na ibugharị ihe nlele nje, nwere ihe anaghị arụ ọrụ nje.
【Specimen ọdabara】 Nasopharyngeal swab, alveolar lavage fluid, wdg.
【Ihe NoNzuko chọrọ maka otu ule, 5-in-1 ma ọ bụ 10-in-1 ule.
【Uru】
- Enweghị nnu guanidine, kwụsiri ike karị
- Nje virus anaghị arụ ọrụ, dị nchebe karịa
- Nchekwa nlele, na-adịgide adịgide
【Njirimara ngwaahịa】
● Nje Virus anaghị arụ ọrụ: Ngwọta nchekwa ahụ nwere protein denaturant nke nnu na-abụghị guanidine, nke nwere ike ime ka nje dị n'ihe nlele ahụ rụọ ọrụ ngwa ngwa.Mgbe ahụ, ọrịa, pathogenicity na ịmụ nwa nke nje, wepụ ọrịa nke abụọ, ma chebe nchekwa nke njem na ndị ọrụ nyocha;
● Ọ dị mfe iji: Mgbe nchịkọta, ndị ọrụ ahụike nwere ike iminye swab ozugbo n'ime tube nlele, ọrụ ahụ dịkwa mfe ma dị mma.Nkọwa nkwakọ ngwaahịa dị iche iche na-egbo mkpa nke ọnọdụ dị iche iche, ma na-emezukwa ihe achọrọ nke "ntụgharị ule 10-in-1 maka 2019-nCoV nucleic acids";
● Mmetụta dị elu: Mgbe nkwenye nke ngwaahịa ngwaahịa, tube sample nwere ike ịbụechekwara na ụlọ okpomọkụ maka awa 24 ma chekwaa na 2-8 ℃ maka ụbọchị 7,na-ezere nsogbu "ụgha ụgha" kpatara nbibi nke sample na ngwaahịa ọdịnala na 4 ℃ ma melite nghọta nchọpụta.
Oge nzipu: Dec-08-2020
